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Bernd Kuebler

Bernd Kuebler

bkuebler@cmrb.eu


El Dr. Bernd Kuebler se graduó en 1995, en Biología Técnica en la Universidad de Stuttgart, Alemania. De 1995 a 1999 hizo el doctorado en el laboratorio del Prof. K. von Figura en el Departamento de Bioquímica II de la Universidad de Göttingen, Alemania. El objetivo de su trabajo fue la purificación, identificación y caracterización de proteasas del factor de crecimiento similar a la insulina, vinculando proteínas (IGFBP) de serum humano.

Desde 1999-2006 trabajó como postdoc en el laboratorio del Prof. T. Braulkes en el Lab Biochemistry Department of Children´s Hospital Hamburg Eppendorf, Alemania, donde continuó sus estudios en varios proyectos sobre el sistema IGF Bioquímica.

Durante este periodo también hizo estancias temporales como científico invitado en el Strangeways Research Laboratory, de Cambridge, Instituto de Patología Molecular, Copenhague, en el Lower Saxony Institute for Peptide Research (IPF), Hannover, y en el Instituto de Biomedicina de la Universidad de Helsinki.

Posteriormente, de 2007-2010, trabajó en el Centro de Regulación Genómica (CRG) de Barcelona y en febrero de 2011 se incorporó al CMRB, donde está trabajando en la generación y caracterización de células madre pluripotentes inducidas (iPS).



Publicaciones seleccionadas

  • Kuebler B, Aran B, Miquel-Serra L, Muñoz Y, Ars E, Bullich G, Furlano M, Torra R, Marti M, Veiga A, Raya A. (2017) Integration-free induced pluripotent stem cells derived from a patient with autosomal recessive Alport syndrome (ARAS). Stem Cell Res 25: 1-5. Epub 2017, Sep 14.
  • Kuebler B, Aran B, Miquel-Serra L, Muñoz Y, Ars E, Bullich G, Furlano M, Torra R, Marti M, Veiga A, Raya A. (2017) Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS) Stem Cell Res 25: 291-295. Epub 2017, Sep 9.
  • Miquel-Serra L, Duarri A, Muñoz Y, Kuebler B, Aran B, Costa C, Martí M, Comabella M, Malhotra S, Montalban X, Veiga A, Raya A. (2017) Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines. Stem Cell Res. 24:155-159. Epub 2017, Jun 7.
  • Paul A. De Sousa, Rachel Steeg, Elisabeth Wachter, Kevin Bruce, Jason King, Marieke Hoeve, Shalinee Khadun, George McConnachie, Julie Holder, Andreas Kurtz, Stefanie Seltmann, Johannes Dewender, Sascha Reimann, Glyn Stacey, Orla O'Shea, Charlotte Chapman, Lyn Healy, Heiko Zimmermann, Bryan Bolton, Trisha Rawat, Isobel Atkin, Anna Veiga, Bernd Kuebler, Blanca Miranda Serano, Tomo Saric, Jürgen Hescheler, Oliver Brüstle, Michael Peitz, Cornelia Thiele, Niels Geijsen, BjørnHolst, Christian Clausen, Majlinda Lako, Lyle Armstrong, Shailesh K. Gupta, Alexander J. Kvist, Ryan Hicks, Anna Jonebring, Gabriella Brolén, Andreas Ebneth, Alfredo CabreraSocorro, Patrik Foerch, Martine Geraerts, Tina C. Stummann, Shawn Harmon, Carol George, Ian Streeter, Laura Clarke, Helen Parkinson, Peter W. Harrison, Adam Faulconbridge, Luca Cherubin, Tony Burdett, Cesar Trigueros, Minal J Patel, Christa Lucas, Barry Hardy, Rok Predan, Joh Dokler, Maja Brajnik, Oliver Keminer, Ole Pless, Philip Gribbon, Carsten Claussen, Annett Ringwald, Beate Kreisel, Aidan Courtney, Timothy E. Allsopp (2017) Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience. Stem Cell Res 20: 105-114. Epub 2017, Mar 7.
  • Vitaloni M, Pulecio J, Bilic J, Kuebler B, Laricchia-Robbio L, Izpisua Belmonte JC. (2013) MicroRNAs Contribute to iPSC-Somatic Donor Memory. J Biol Chem 289(4):2084-98. Epub 2013 Dec 5.
  • Marti M, Montserrat N, Pardo C, Mulero L, Miquel-Serra L, Rodrigues AM, Andrés Vaquero J, Kuebler B, Morera C, Barrero MJ, Izpisua Belmonte JC. (2013) Mcad-mediated intercellular interactions activate satellite cell division. J Cell Sci 126 (Pt 22):5116-31. Epub 2013 Sep 17.
  • Barrero MJ, Sese B, Kuebler B, Bilic J, Boue S, Martí M, Izpisua Belmonte JC. (2013) Macrohistone Variants Preserve Cell Identity by Preventing the Gain of H3K4me2 during Reprogramming to Pluripotency. Cell Rep 3(4):1005-11. Epub 2013 Mar 28.
  • Nascimento EM, Cox CL, Macarthur S, Hussain S, Trotter M, Blanco S, Suraj M, Nichols J, Kübler B, Benitah SA, Hendrich B, Odom DT, Frye M. (2011) The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nat Cell Biol 13(12): 1395-405.
  • Mejetta S, Morey L, Pascual G, Kuebler B, Mysliwiec MR, Lee Y, Shiekhattar R, Di Croce L, Aznar Benitah S. (2011) Jarid2 regulates mouse epidermal stem cell activation and differentiation. EMBO J 30(17): 3635-46.
  • Luis NM, Morey L, Mejetta S, Pascual G, Janich P, Kuebler B, Roma G, Nascimento E, Frye M, Di Croce L, Benitah Sa. (2011) Regulation of human epidermal stem cell proliferation and senescence requires polycomb- dependent and -independent functions of Cbx4. Cell Stem Cell 9(3): 233-46.
  • Uribesalgo I, Buschbeck M, Gutiérrez A, Teichmann S, Kuebler B, Caballero JM, Roma G, Aznar Benitah S, Di Croce L. (2011) E-box-independent regulation of transcription and differentiation by MYC. Nat Cell Biol 13(12): 1443-9.
  • Shalamanova L, Kubler B, Storch S, Scharf JG, Braulke T. (2008) Multiple post-translational modifications of mouse insulin-like growth factor binding protein-6 expressed in epithelial Madin-Darby canine kidney cells. Mol Cell Endocrinol 295 (1-2): 18-23.
  • Viereck V., Siggelkow H., Braulke T., Scharf J-G., Kübler B. (2007) Alteration of the insulin-like growth factor axis during in vitro differentiation of the human osteosarcoma cell line HOS 58. J Cell Biochem 102 (1): 28-40.
  • Mark S., Kübler B., Höning S., Oesterreicher S., John H., Braulke T., Forssmann W.G., Ständker L. (2005) Diversity of human insulin-like growth factor (IGF) binding protein-2 fragments in human plasma: primary structure, IGF binding properties, and disulfide bonding pattern. Biochemistry 44 (9): 3644-52.
  • Oesterreicher S., Blum W.F., Braulke T., Kübler B. (2005) Interaction of IGF-II with multiple plasma proteins. High-affinity binding of plasminogen to IGF-II and IGF-binding protein-3. Journal of Biological Chemistry 280 (11): 9994-10000.
  • Hammer E., Kutsche K., Haag F., Ullrich K., Sudbrak R., Willig R.P., Braulke T., Kübler B. (2004) Mono-allelic expression of the IGF-I receptor does not affect IGF responses in human fibroblasts. European Journal of Endocrinology 151 (4): 521-529.
  • Schebek-Fürstenberg V., Ständker L., Oppermann M., Müller-Wiefel D.E., Hahn W., Blum W.F., Braulke T., Kübler B. (2004) Characterization of IGF binding polypeptides in plasma of a child with acute renal failure. Pediatric Nephrology 141(12): 1418-25.
  • Scharf J.-G., Dombrowski F., Novosyadlyy R., Eisenbach C., Demori I., Kübler B., Braulke T. (2004) Insulin-like growth factor (IGF)-binding protein-1 is highly induced during acute carbon tetrachloride liver injury and potentiates the IGF-I-stimulated activation of rat hepatic stellate cells. Endocrinology 145(7): 3463-3472.
  • Ständker L., Kübler B., Obendorf M., Braulke T., Forssmann W.-G., Mark S. (2003) In vivo processed fragments of IGF binding protein-2 copurified with bioactive IGF-II. Biochemical and Biophysical Research Communications 304: 708-713.
  • Kübler B., Dräger C., John H., Andag U., Scharf J.-G., Forssmann W.-G., Braulke T., Ständker L. (2002) Isolation and characterization of circulating fragments of the insulin-like growth factor binding protein-3. FEBS Lett 518: 124-128.
  • Zwad O., Kübler B., Roth W., Scharf J-G., Saftig P., Peters C., Braulke T. (2002) Decreased intracellular degradation of IGFBP-3 in cathepsin L-deficient fibroblasts. FEBS Lett 510: 211-215.
  • Storch S., Kübler B., Höning S., Ackmann M., Zapf J., Blum W., Braulke T. (2001) Transferrin binds IGFs and effects binding properties of IGFBP-3. FEBS Lett 509: 395-398.
  • Shalamanova L., Kübler B., Scharf J-G., Braulke T. (2001) MDCK cells secrete neutral proteases cleaving insulin-like growth factor-binding protein-2 to –6.  Am J Physiol Endocrinol Metab 281: E1221-1229. 
  • Shalamanova L., Kübler B., Scharf J-G., Braulke T. (2000) Ligand blotting: Iodinated vs biotinylated IGF. Growth Hormone IGF Res 10: 294.
  • Putzer P., Götz W., Gross M., Kübler B., Scharf J.-G., Schuller A.G.P., Hartmann, H., Braulke T. (1998) Mouse insulin-like growth factor binding protein-6: Expression, purification, characterization and histochemical localization. Mol Cell Endocrinol 137: 69-78.
  • Kübler B., Cowell S., Zapf J., Braulke T. (1998) Proteolysis of insulin-like growth factor binding proteins by a novel 50-kilodalton metalloproteinase in human pregnancy serum. Endocrinology 139: 1556-1563.
  • Claussen M., Kübler B., Wendland M., Neifer K., Schmidt B., Zapf J., Braulke T. (1997) Proteolysis of insulin-like growth factor (IGF) and IGF binding proteins by cathepsin D. Endocrinology 138: 3797-3803.



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